Fast and Automatic Mapping of Disulfide Bonds in a Monoclonal Antibody using SYNAPT G2 HDMS and BiopharmaLynx 1.3
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چکیده
Furthermore, the assignment of disulfide linkages sometime is inconclusive when a protein contains multiple cysteines due to the significantly increased number of possible disulfide-bonded peptide isomers. For example, there are totally 105 possible disulfide-bond pairing schemes for a protein containing eight cysteine residues (while an IgG1 mAb typically has 32 or more cysteines in its two light chains and two heavy chains).
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تاریخ انتشار 2011